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Scientist III
Job Description
Scientist III
Protein Purification
Duties:
We are seeking a talented and experienced Scientist III who will conduct protein purification and analytics to support development of protein biotherapeutics in a fast-paced research environment. This person will be primarily responsible for designing and executing protein purification and analytics as well as, analyzing and interpreting assays and production data to understand inherent protein attributes and physicochemical stability. The ideal candidate will be an analytical thinker with creative problem-solving skills;a quick learner who takes the initiative and manages workflows for several projects with enthusiasm and resourcefulness;an effective collaborator, working with different functional areas to enhance project knowledge.
Plan, coordinate and perform purification of biotherapeutic proteins using standard chromatography methods (Affinity capture, SEC, CEX, AEX, MMC, HIC). Troubleshoot complex systems as necessary through process development.
Perform standard analytical techniques to assess protein purity, stability and quality (HPLC-SEC, SDS-PAGE, CGE, DLS)
Evaluate new technologies and process changes to improve the productivity, quality and consistency of standard platform processes.
Perform stage appropriate purification assessments, including screening of protein behaviors and attributes in relevant process conditions, interpret and present findings to other Biotherapeutics Discovery colleagues.
Develop Client analytical methodologies and evaluate new technologies to improve purification team capabilities
Contribute to broader research goals in areas related to protein biochemistry aimed at developing a better understanding of therapeutic protein structure, stability, dynamics, and function.
Provide protein biochemistry and protein purification support for other scientists across Biotherapeutics Discovery;perform other tasks as assigned by manager in a timely manner.
Improve data quality, consistency, and throughput using automation and analysis scripts. Identify opportunities for innovative automation strategies to streamline operations and boost workflow efficiency.
Comply with all applicable regulations, perform all work in a safe manner;maintain proper records in accordance with SOP s and policies.
Skills:
horough understanding of cell culture basics.
Hands-on experience in cell culture (mammalian) and exceptional aseptic techniques.
Strong attention to details and excellent organizational skills.
Able to learn quickly and excel in a past-faced work environment;self-motivation, adaptability and positive attitude.
Hands-on experience in handling automation/HTP antibody expression is a plus
Ability to work in plate based formats (24 well, 96 well)
Knowledge of DNA and molecular biology including basic DNA quality assessments
Apply knowledge of basic theory and scientific principles
Concise and accurate reporting of technical data and information
Ability to troubleshoot and multi-task
Ability to work in team-oriented environment
Excellent written and verbal communication skills
Protein Purification
Duties:
We are seeking a talented and experienced Scientist III who will conduct protein purification and analytics to support development of protein biotherapeutics in a fast-paced research environment. This person will be primarily responsible for designing and executing protein purification and analytics as well as, analyzing and interpreting assays and production data to understand inherent protein attributes and physicochemical stability. The ideal candidate will be an analytical thinker with creative problem-solving skills;a quick learner who takes the initiative and manages workflows for several projects with enthusiasm and resourcefulness;an effective collaborator, working with different functional areas to enhance project knowledge.
Plan, coordinate and perform purification of biotherapeutic proteins using standard chromatography methods (Affinity capture, SEC, CEX, AEX, MMC, HIC). Troubleshoot complex systems as necessary through process development.
Perform standard analytical techniques to assess protein purity, stability and quality (HPLC-SEC, SDS-PAGE, CGE, DLS)
Evaluate new technologies and process changes to improve the productivity, quality and consistency of standard platform processes.
Perform stage appropriate purification assessments, including screening of protein behaviors and attributes in relevant process conditions, interpret and present findings to other Biotherapeutics Discovery colleagues.
Develop Client analytical methodologies and evaluate new technologies to improve purification team capabilities
Contribute to broader research goals in areas related to protein biochemistry aimed at developing a better understanding of therapeutic protein structure, stability, dynamics, and function.
Provide protein biochemistry and protein purification support for other scientists across Biotherapeutics Discovery;perform other tasks as assigned by manager in a timely manner.
Improve data quality, consistency, and throughput using automation and analysis scripts. Identify opportunities for innovative automation strategies to streamline operations and boost workflow efficiency.
Comply with all applicable regulations, perform all work in a safe manner;maintain proper records in accordance with SOP s and policies.
Skills:
horough understanding of cell culture basics.
Hands-on experience in cell culture (mammalian) and exceptional aseptic techniques.
Strong attention to details and excellent organizational skills.
Able to learn quickly and excel in a past-faced work environment;self-motivation, adaptability and positive attitude.
Hands-on experience in handling automation/HTP antibody expression is a plus
Ability to work in plate based formats (24 well, 96 well)
Knowledge of DNA and molecular biology including basic DNA quality assessments
Apply knowledge of basic theory and scientific principles
Concise and accurate reporting of technical data and information
Ability to troubleshoot and multi-task
Ability to work in team-oriented environment
Excellent written and verbal communication skills
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